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非侵入性技术瞬时鉴定男性精子活性  发帖心情 Post By:2010/6/11 11:41:21

来自鲁尔波鸿大学的科学家开发了一种非侵入性技术,通过采集光谱化学指纹图谱数秒钟内便可区分可生育精子细胞和不可生育精子细胞。该方法具有成为一种全新的生育技术和测试方案的潜力,它并不只依靠形态特征,而且还利用化学签名。

该研究结果发表在The Analyst杂志上,并在5月份的Highlights in Chemical Biology和英国皇家化学学会Chemistry World杂志的新闻版被突出报道。

大自然用与赛汽车相似的方式来优化精子细胞的主要功能。这些细胞由亚细胞器组成,细胞器包含如线粒体。线粒体就像发电站为精子的运动和活力提供能量--这是受精过程的一个关键因素。该研究小组发现,虽然精子细胞的损伤在形式和形态学上不可见,但却可在分子水平呈现出来。这强调,除了形态学这一世卫组织男科学检验手册中为人类精子生育能力分级的严格指标,现在有可能也有必要将化学指标也纳入分级标准中以改进这项检查。

鲁尔波鸿大学的研究人员得到了一份详细的3D化学地图,并且不再需要引入额外的标签和标记。拉曼显微光谱根据基于分子振动特征的光谱指纹来检测亚细胞成分。人类精子中不同的细胞器可用化学性特异的拉曼光谱分别显示出来。除了光学和形态学图像之外,现在可以直接描绘人类精子细胞个体的化学组分。

这一发现可能有助于发展为健康可育和损坏不育的人类精子细胞分级的新标准。自从世界范围内人类男性精子计数降低到了50年前的50%这一警戒线以来,调查人类生育细胞器的任务变的很紧急。

为活细胞高清照相的创新性分光镜和显微镜方法的研发是物理化学II主席(Martina Havenith-Newen教授)的研究领域之一。Konrad Meister, Diedrich A. Schmidt和Erik Bründermann用拉曼共聚焦显微镜为人类精子细胞的细胞器进行三维成像和鉴定,从而在分子水平揭示不育的根源。这项研究所用的拉曼显微镜由联邦教育与研究部资助(BMBF 05KS7PC2)。


http://www.sciencedaily.com/releases/2010/05/100518064608.htm

Scientists from Ruhr-Universit?t Bochum have developed a non-invasive technique that within seconds can distinguish healthy fertile and infertile sperm cells by collecting the spectral chemical fingerprint. The method has the potential for a novel fertility technology and a test scheme which does not only rely on morphological characteristics, but also utilizes chemical signatures.

The findings are published in the journal The Analyst, and are also featured in Highlights in Chemical Biology and in the news section of Chemistry World of the Royal Society of Chemistry in May.

Nature has optimized the main function of sperm cells in a similar way to race cars. These cells consist of sub-cellular organelles that contain, for example, mitochondria. Mitochondria are the power stations supplying energy for sperm motion and mobility -- one of the crucial factors in fertility. The research team has shown that cellular damage on a molecular level in mitochondria can be present although changes in form and morphology are not visible. This stresses that besides morphology, which is a strict criterion by the WHO Manual for Andrology Laboratories to classify human sperm fertility, it is now possible and necessary to improve the test criteria by incorporating chemical signatures.

The RUB researchers obtained detailed 3D chemical maps which do not require the introduction of additional labels or markers. The Raman micro-spectroscopy used in this study detects sub-cellular components using the spectral fingerprints of molecules based on their characteristic vibrations. Different organelles within human sperm are visualized by their chemically unique Raman spectra. In addition to optical and morphological images, it is now possible to directly image the chemical constituents of individual human sperm cells.

This discovery may contribute to the development of new standards for the classification of healthy fertile and damaged infertile human sperm cells. Since the sperm count of human males around the world has dropped an alarming 50 per cent of what it was more than 50 years ago, there is an urgency to investigate human fertility organelles.

The development of innovative spectroscopic and microscopic methods for high resolution imaging of living cells is one of the research areas at the Chair of Physical Chemistry II (Prof. Dr. Martina Havenith-Newen). The work of Konrad Meister, Diedrich A. Schmidt and Erik Bründermann uses confocal Raman microscopy for detailed 3D imaging and identification of organelles of human sperm cells to reveal the origin of infertility on the molecular level. The funding for the Raman microscope used in this study was supported by the Federal Ministry of Education and Research (BMBF 05KS7PC2).



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